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EAS Advanced Course in Cardiometabolic Phenotyping in animal models - Preliminary Programme

Monday July 01, 2019 Arrivals in the afternoon
  from 14.00 -  Check-in at Trinity Hall, Wychfield Site
  18.30-21.00  Informal Welcome Dinner 
Tuesday July 02, 2019   

07.30-08.15 Breakfast at Trinity Hall, Wychfield Site
  08.30 Coach departing from Trinity Hall, Wychfield Site
  09.00-09.20 Coffee and Registration
  09.20-09.30  Welcome address
Course Director Prof Antonio Vidal-Puig, Cambridge, UK
  09.30-11.00  Myocardial Biology
Dr Thomas Krieg and group, Cambridge, UK 

11.00-11.30  Coffee & tea break
  11.30-13.00  Vascular Biology / Inflammation
Prof Ziad Mallat and group, Cambridge, UK
Dr Andrew Sage, Cambridge, UK
Dr Meritxell Nus, Cambridge, UK
Dr Xuan Li, Cambridge, UK

13.00-14.00  Lunch
  14.00-14.15  Walk to animal facility 
  14.15-14.45  Tour of Animal Phenotyping Facility
  14.45-17.15 Workshops, each of 30 minutes in smaller groups
    1. Metabolic dissection
Disease Model Core (DMC)
Vidal-Puig group, Cambridge, UK 
    2. CLAMPS
DMC
Vidal-Puig group, Cambridge, UK 
    3. "Ex vivo langedorff-perfused heart preparation"
Dr Thomas Krieg and group, Cambridge, UK 
    4. Echocardiogram analysis
Dr Thomas Krieg and group, Cambridge, UK 
    5. Aorta and carotid artery perfusion and dissection
Prof Ziad Mallat group, Cambridge, UK 
  17.45 Coach departing to Trinity Hall, Wychfield Site  
Wednesday July 03, 2019   
  07.30-08.00 Breakfast at Trinity Hall, Wychfield Site 
  08.15  Coach departing from Trinity Hall, Wychfield Site
  08.45-09.30  Assessment of in vivo Insulin Sensitivity
"Assessing glucose metabolism in mice"
Dr Mark Evans, Cambridge, UK 
  09.30-10.15  Measuring energy expenditure in rodents
"The principles of assessing energy balance and metabolic rate in mice"
Dr Samuel Virtue, Cambridge, UK 
  10.15-10.45  Coffee & tea break 
  10.45-13.00  Computer practical session for analysis of data 
    Part A 
    1. How to check if your measured data is correct 
    2. How to analyze and interpret tracer kinetic data 
    3. How to determine insulin sensitivity 
    Part B 
    1. Quality control of indirect calorimetry data
    2. How to analyze energy expenditure and food intake data 
    3. How to calculate energy balance 
    4. Analysis of metabolic rate using different methods 
  13.00-14.00  Lunch 
  14.00-16.00  Computer Practical, Analysis of Athersclerotic plaques
Dr Andrew Sage, Cambridge, UK
 
  16.00-16.30  Coffee & tea break 
  16.30-17.30  How to make mice more human
Prof Barbara Cannon, Stockholm, Sweden 
  17.45  Coach departing to Trinity Hall, Wychfield Site 
  19.15  Coach departing for the Offical Dinner 
  19.30-23.00 Drinks Reception & Official Dinner
  20.00-20.30 Pre-dinner lecture: The ELH, the EU and the 3R's. As clear as mud!!
Dr Martin Vinnell, Cambridge, UK
  23.00  Coach departing to Trinity Hall, Wychfield Site 
Thursday July 04, 2019   

07.30-08.15  Breakfast at Trinity Hall, Wychfield Site
Check-out! Your luggage can be stored at the reception
  08.30  Coach departing from Trinity Hall, Wychfield Site 
  09.00-11.00 Large animals as model organisms to study cardiovascular disease
Tour of the Barcroft Centre
Dr Kim Botting, Cambridge, UK

Participants will be split into smaller groups and each group will spend 15-20 minutes at a given station, The stations will be as follows:
    1. Tour of the surgical facilities 
    2. Demonstration of the chronic hypoxia chicken incubators, bespoke sheep chambers and our wireless in vivo cardiovascular data acquisition system 
    3. Live isolated heart preparation on a Langendorff apparatus and tour of the chicken barn with adult offspring 
    4. Live in vitro wire myography with isolated blood vessels and tour of the nitrogen generator plant 
    5. Written exercise 
  11.30 Coach departing Barcroft Centre to Institute of Metabolic Sciences
  12.00-13.00   Lunch
  13.00-14.00  The use of avian and ovine models in cardiovascular research
Dr Kim Botting, Cambridge, UK 
  14.00-15.00  Assessing vascular repair and angiogenesis in mice
Dr Richard Cubbon, Leeds, UK
  15.00-15.30  Coffee & tea break 
  15.30-16.30  Modelling Experimental data from Animal Experiments
Dr Gavin Jarvis, Cambridge, UK
  16.30-16.50 Closing words
Course Director Prof Antonio Vidal-Puig, Cambridge, UK
  17.00  Coach departing to Trinity Hall, Wychfield Site 
    Departure 
15:00-15:30 Coffee Break
15:00-15:30 Coffee Break
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