EAS Advanced Course in

Metabolomics for Clinical Research

Helsinki, Finland, September 21-23, 2010

Programme: DAY 1

09:00-09:15

Welcome and general introduction to the course

09:15-10:15

Lecture: Matej Orešič
Metabolomics and lipidomics, an introduction
10:15-10:45

Lecture: Tuulia Hyötyläinen
Sample preparation for metabolomics
10:45-11:30

Lecture: Tuulia Hyötyläinen
Platforms for global metabolomics
11:30-12:30 Lunch
12:30-14.00

Lecture, Jan Borén / Marja-Riitta Taskinen
Introduction to studies of lipoprotein metabolism: overview of methodology 

14:00-17:00

Laboratory work: Heli Nygren, Maarit Kivilompolo and Ismo Mattila

Sample preparation and setting up the runs for UPLC-QTOFMS and GC×GCTOF/MS. The samples are (1) selected lipoprotein fractions from a kinetic study and (2) small series of liver samples from a mouse study (ob/ob vs. WT).
 

Programme: DAY 2

09:00-10:00

Lecture: Gopal Peddinti
Overview of bioinformatics for metabolomics

10:00-12:00

Lecture and computer work: Sandra Castillo
Processing of the data using MZmine 2: peak detection, alignment, tracerdetection, normalization, quality control
12:00-13:00 Lunch
13:00-15:00

Lecture and computer work: Matej Orešič, Sandra Castillo, Gopal Peddinti

Preliminary statistical analysis and identification of metabolic biomarkers

15:00-16:00

Lecture: Tuulia Hyötyläinen
Metabolite identification -principles and methods

 
16:00-17:00      Laboratory work: Heli Nygren
Setup of MSMS run examples for identification

Programme: DAY 3

09:00-12:00

Computer work: Matej Orešič, Gopal Peddinti, Jing Tang, Tuulia Hyötyläinen, Heli Nygren

Data analysis, biomarker identification, and interpretation of the results
12:00-13:00     Lunch
13:00-

Discussion and Conclusions